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(?) Enter a search word or phrase (left box), optionally select an appropriate field (middle), and hit the search button or press enter. This search engine is NOT case sensitive.

Use wild characters " ? " and " * " for efficient search:
  • ? : A single character. E.g., trehalose?phosphatase covers both trehalose phosphatase and trehalose-phosphatase, of which, only the latter fits the nomenclature used in this database.
  • * : Any number of characters. E.g.,tre-* brings all genes from trehalase family; coenzyme*A covers coenzyme A, coenzyme-A, and coenzymeA.
Network Biomass WormPaths
FBAFlux Balance Analysis PEAPathway Enrichment Analysis
Gene Overview
Name ..................................................... : aman-2
Sequence ID ..................................................... : F58H1.1
Status (?) Can be one of the following two: Curated (manually annotated or based on a curated SACURE result), or Not curated (SACURE result reported without manual check). ..................................................... : Curated
KO (?) The KEGG orthology group (KO) that is associated with this gene. ..................................................... : K01231MAN2; alpha-mannosidase II [EC:3.2.1.114]
Enzymes in the model (?) Enzymes that are part of the current metabolic network model. ..................................................... : 3.2.1.114names: mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase;mannosidase II;exo-1,3-1,6-alpha-mannosidase;alpha-D-mannosidase II;alpha-mannosidase II;alpha1-3,6-mannosidase;GlcNAc transferase I-dependent alpha1,3[alpha1,6]mannosidase;Golgi alpha-mannosidase II;ManII;1,3(1,6)-alpha-D-mannosidase;1,3-(1,6-)mannosyl-oligosaccharide alpha-D-mannohydrolase
Other enzymes (?) Enzymes that are associated with this gene in the WormFlux database, but not included in the current network model. ..................................................... : None
Model reactions (?) Reactions that are part of the current metabolic network model.

See the bottom of this page for a table of reactions.
.....................................................
: RCC0038, RCC0039
Relevant model reactions (?) Model reactions that follow from the KEGG reactions associated with this gene, but that exclude this gene due to differences in reaction specificity or localization compared to the predictions for this gene. ..................................................... : ERROR
Other reactions (?) KEGG reactions that are associated with this gene in the WormFlux database, but not included in the current network model. ..................................................... : None

In WormPaths (?) Pathway maps where this gene is represented are listed here. ..................................................... : N-Glycan biosynthesis
► | Gene-KO-Enzyme-Reaction Associations (click to view)▼ | Gene-KO-Enzyme-Reaction Associations (click to hide)
► | SACURE Summary (click to view)▼ | SACURE scores (click to hide)

Score table (?) Association scores for KOs best matching to the query gene according to each annotation predictor, and for the accepted KO if there is any (rightmost columns).

The predictors are ordered in decreasing weight of contribution to the decision-making process in SACURE.

See above for the final decision on the annotation of this gene.

Predictor Best scoring KOs Best score K01231 ERROR
KEGG (?) Score represents the annotation in KEGG database by June 2014.

Takes a value of either 0 (KO is not associated with the query gene) or 1 (KO is associated with the query gene).
K01231 1 1 ERROR
Cluster (?) Score represents the degree of relatedness of a KO to the query gene based on the clustering in the phylogenetic tree of protein sequences (see below for the tree).

Takes values from 0 to 1.
K01231 0.67 0.67 ERROR
myKEGG (?) Score represents the degree of relatedness of a KO to the query gene based on the Smith-Waterman alignment of protein sequence with most similar sequences from all organisms in the KEGG database.

Takes values from 0 to 1.

KOunknown indicates the score for sequences without any KO association in KEGG database.
K01231 0.99 0.99 ERROR
Tree (?) Score shows the degree of relatedness of a KO to the query gene based on the density of this KO in the phylogenetic tree of protein sequences (see below for the tree).

Takes values from 0 to 2 as the score is normalized to the second best KO.

KOunknown indicates the score for sequences without any KO association in KEGG database.
K01231 1.96 1.96 ERROR
KOG (?) Score indicates the absence or presence of a KO in association with one of the orthologs of the query gene according to a eukaryotic orthology database independent of KEGG (see HELP in the upper menu for details).

Takes a value of either 0 (absent) or 1 (present).
K01231 1 1 ERROR
WormBase (?) Score represents matching between the KEGG descriptions of a KO (KO name and all related enzyme names) and the descriptions of the query gene in WormBase (gene overview and protein domains).

Value is increased by 0.5 for every perfect word match and reduced by 0.1 for each character interruption. A non-matching word always creates a 0 score.

WormBase description used for this gene was:

"Alpha mannosidase, middle $ Glycoside hydrolase, family 38, central $ Glycoside hydrolase, family 38, core $ Glycosyl hydrolases 38, C-terminal $ Glycosyl hydrolases family 38 C-terminal $ Glycosyl hydrolases family 38 N-terminal $$ aman-2 encodes an alpha-mannosidase II, homologous to human MAN2A1 (OMIM:154582) and MAN2A2, that is required for normal N-glycan structures and predicted to act in the Golgi; AMAN-2 is predicted to mature glycoproteins by removing mannose residues from their N-linked oligosaccharides; translational fusions of AMAN-2 with GFP are subcellularly localized in a pattern consistent with Golgi function; aman-2 is broadly expressed in larvae; in adults, aman-2 is most strongly expressed in the digestive system (gut wall, pharynx and grinder), hypodermal cells, and neurons; aman-2 mutants have N-glycans not seen in normal worms, such as Hex5-7HexNAc2-3Fuc2Me, along with hybrid oligosaccharides, while having reduced paucimannosidic and trifucosylated glycans, and completely lacking tetrafucosylated ones; AMAN-2 contains three predicted N-glycosylation sites, one of which (Asn-320) is known to be occupied in vivo; Cu(II) was observed to completely inhibit AMAN-2, which it also does with mouse and Drosophila alpha-mannosidase II; the pH optimum of AMAN-2 is pH 5.5-6.0; several other ionic conditions (e.g., Co[II] or EDTA) have little effect on AMAN-2; aman-2(tm1078) mutants are superficially wild-type, but mutant extracts completely lack alpha-mannosidase II activity; aman-2(tm1078) mutants have reduced, but not absent, anti-horseradish peroxidase and phosphorylcholine epitopes."
K01231 K01191
and 8 more...All best scoring KOs: K01231, K01191, K15524, K10788, K12550, K11188, K00430, K01230, K12311, K12312
1.0 1.0 ERROR
Overall (?) Overall score from SACURE that represents the logistic-regression-based probability of acceptance of a KO in association with the query gene. K01231 1.000 1.000 ERROR
► | Localization scores (click to view)▼ | Localization scores (click to hide)

Score table (?) Localization scores for proteins encoded by the query gene.

Each predictor is used to determine whether the protein is localized to mitochondria or other compartment.

Together with other genes and other predictors, these scores were used in the determination of the subcellular location of reactions associated with the query gene (see below for the reactions).

Predictor Mitochondrial Other
Mitoprot (?) Score represents the predicted probability that the protein is targeted to mitochondria (mitochondrial score) or not (other score) according to Mitoprot (click to reach).

If multiple isofoms of the same protein are available the highest scores are shown for each compartment.
0.07 0.93
MitoMiner (?) Score evaluates the evidence for mitochondrial targeting based on the orthologs in 12 eukaryotic model organisms that are in the MitoMiner database (click to reach).

If clear orthologs for the query gene are available in MitoMiner organisms, the score takes a value between 0 and 1 as a function of the ratio of orthologs that have hits (contributes to the mitochondrial score) or no hits (determines the other score) in the database. Mitochondrial score is also a function of the strength of the evidence for the orthologs found in the database (experimental evidence is given a higher weight than theoretical evidence during scoring).
0.00 1.00
UniProt (?) Score shows the subcellular localization of proteins encoded by this gene as reported in UniProt (click to reach) in October 2014.

If a report is available, the score takes a value of either 0 (not reported to be localized to the pertaining compartment) or 1 (reported to be localized to the pertaining compartment).
Not available Not available
Org. DB (?) Score shows the subcellular localization of proteins encoded by this gene as reported in Organelle Database (click to reach).

If a report is available, the score takes a value of either 0 (not reported to be localized to the pertaining compartment) or 1 (reported to be localized to the pertaining compartment).
Not available Not available
► | Phylogenetic tree (click to view)▼ | Phylogenetic tree (click to hide)
K01191 BAC:oni Osc7112_0642 (R) K01191 FUN:aje HCAG_04277 (R) K01191 ARC:mfv Mfer_0546 (R) K01231 ART:tca 660728 (R) K01231 DME:dme alpha-Man-II (R) K01231 MAM:mdo MAN2A1 (R) K01231 VER:pss MAN2A1 (R) K01231 HSA:hsa MAN2A1 (R) K01231 INV:cin 100180083 (R) K01231 ATH:ath GMII (R) K01231 PLA:csv MAN2A2 (R) KO-TBD CEL:cel aman-2 (SUBJECT) K01231 NEM:cbr Cbr-aman-2 (R) KOunknown CEL:cel aman-3 (P) K12311 CEL:cel aman-1 (P) K01231 PRO:ngr NAEGRDRAFT_57722 (R) 0.1
Sequence labels indicate KO, taxonomy or model organism, organism name (if taxonomic group), and gene name, respectively. CEL, C. elegans; HSA, H. sapiens; DME, D. melanogaster; ATH, A. thaliana; SCE, S. cerevisiae; BAC, bacteria; ARC, archaea; PRO, protists; FUN, fungi; PLA, plants; INV, invertebrates; NEM, nematodes; ART, arthropods; VER, vertebrates; MAM, mammals. Parenthetical information for organisms other than CEL indicates whether the genes are introduced as best matches (B) or reciprocal best hits (R) to the query gene. Parenthetical information for CEL indicates whether the genes are introduced as paralogues (P) of the query gene or as reciprocal best matches to one of the other organisms in the tree (B). Organism abbreviations are from KEGG.
Reactions
ID Enzyme Reactants Products Genes (?) Multiple genes are separated by OR (I) or AND (&).

I indicates that genes are redundant within the group (e.g. paralogs encoding the same enzyme).
& indicates that genes are non-redundant within the group (e.g. genes encoding different subunits of the same enzyme).

If there are multiple groups of genes, as in an enzyme complex with some enzymes associated with multiple genes, or in a case where the same enzyme is associated with different orthology groups with paralogs, each group with multiple genes is shown in parentheses.
Pathway
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